Cell Culture Workshop

1-Week Hands-on Workshop on Human/Cancer Cell Culture Techniques & MTT Assay, New Delhi, India

Venue: ICSCCB, First Floor, Khasra 132, Plot 4, IGNOU Main Road, Near Saint Robin Public School, Neb Sarai, Saket, New Delhi – 110068, India (Nearest Metro Station is Saket on the Yellow Line)

You can check lab photos on our posts at: https://www.linkedin.com/company/icsccb and https://www.linkedin.com/in/dr-sheo-mohan-singh

Organizer:
Prof. Dr. Sheo Mohan Singh
MSc(UK), PhD(Germany), PDF(USA,UK,Germany)
Director, ICSCCB, New Delhi, India

Workshop Dates (Timings 10:00am-6:00pm):
24 – 30 Jun. 2024   (FULL)
22 – 28 Jul. 2024   (2 Seats Available)
22 – 28 Aug 2024   (Seats Available)

Workshop Topics:
The following techniques are done real hands-on by each candidate using human embryonic kidney 293T and human cancer cell lines:

1. MTT Assay for cell proliferation/cytotoxicity
2. Cell culture techniques, passaging and maintenance of human cancer cell lines
3. Media preparation and counting of cells
4. Cryopreservation: Freezing & thawing of human cancer cell lines
5. Genomic DNA isolation from cancer cell lines,
6. Genomic DNA estimation
7. Genomic DNA agarose gel electrophoresis
8. Total RNA extraction from human cancer cell lines
9. cDNA synthesis from RNA (Reverse Transcription),
10. Polymerase Chain Reaction from cDNA (Reverse Transcription-PCR)
11. Agarose gel electrophoresis of PCR product
12. Total protein extraction from human cancer cell lines
13. Protein estimation
14. SDS-PAGE electrophoresis of extracted proteins
15. Coomassie blue staining of protein gel

This workshop is designed for beginners of all ages who wants to become confidant in lab techniques. This is a practical hands-on workshop to train the candidates in animal cell culture, molecular biology techniques and MTT assay using the human embryonic and cancer cell lines for various experiments. It is supported by lectures on cell culture techniques, molecular biology assays, MTT assay, gene/protein expression/interaction networks in hematopoietic stem cells (HSCs) and cancer cells.

Only 12 seats per batch so please register early to confirm your participation. We will update on the website when seats are full in a batch. Each participant will do all techniques real hands-on.

Registration Fees (food & accommodation is not included):
Rs. 9500 + 18% GST, Rs.1710 (Total Rs. 11210). Same fees for International candidates.

Registration fee is non-refundable (because of limited seats to maintain the high quality of training). If we cannot provide the seat then the fees will be refunded. One time date change will be allowed under special circumstances.

Methods of Registration:
After depositing the fees by payment methods below, please fill the following online application form: https://forms.gle/NiAgyRbaDQy6RP466 and upload the payment proof/screen shot in the form. If there is any problem in filling the form, contact us by email: icsccb2012@gmail.com

Bank Transfer: You can pay to following bank account:
Account name: International Centre for Stem Cells CAB
Bank name: HDFC Bank, Aundh Branch
IFSC Code:HDFC0000052
MMID: 9240137
Account type: Current Account
Account number: 50200032963132

Payment by any UPI method e.g. Google Pay, PhonePe, Paytm etc:
You can make payment to the following ICSCCB UPI Id: 9545089202@upi

WORKSHOP CERTIFICATE will be issued to all participants on last day.

Eligibility:
Current or past Students in UG, PG and PhD programs, medical students, doctors, lecturers, scientists, industry members in the field of Biological Sciences, Biotechnology, Bioinformatics, Life Sciences, Medical Sciences, Pharmaceutical Sciences, Chemical Sciences and related subject areas who are interested in learning hands-on laboratory techniques.

Accommodation:
Accommodation should be booked in nearby PG hostels/guesthouses or hotels directly by the candidate. For more information and any help in finding accommodation please email to icsccb2012@gmail.com.

For enquiries please email or call:
Dr. Sheo Mohan Singh
Email: icsccb2012@gmail.com
Tel No: +91-7798490002