1-Week Hands-on Workshop on Human/Cancer Cell Culture Techniques & MTT Assay, New Delhi, India
Venue: ICSCCB, First Floor, Khasra 132, Plot 4, IGNOU Main Road, Near Saint Robin Public School, Neb Sarai, Saket, New Delhi – 110068, India (Nearest Metro Station is Saket on the Yellow Line)
Prof. Dr. Sheo Mohan Singh
MSc(UK), PhD(Germany), PDF(USA,UK,Germany)
Director, ICSCCB, New Delhi, India
Workshop Dates (Timings 10:00am-6:00pm):
20 – 26 Oct. 2023 (Seats available)
10 – 16 Nov. 2023 (Seats available)
24 – 30 Dec. 2023 (Seats available)
08 – 14 Jan. 2024 (Seats available)
This is designed for beginners who wants to learn from scratch. This is a real hands-on workshop to train the candidates in animal cell culture, molecular biology techniques and MTT assay using the human/cancer cell lines for various experiments. It is supported by lectures on basic cell culture techniques, molecular biology assays, MTT assay, and gene/protein expression & interaction networks in hematopoietic stem cells (HSCs) and cancer cells.
The following techniques are done real hands-on by each candidate using human embryonic kidney 293T and human cancer cell lines:
1. Cell culture techniques, passaging and maintenance of human cancer cell lines
2. Media preparation
3. Cryopreservation: Freezing & thawing of human cancer cell lines
4. Genomic DNA isolation from cancer cell lines,
5. Genomic DNA estimation
6. Genomic DNA agarose gel electrophoresis
7. Total RNA extraction from human cancer cell lines
8. cDNA synthesis from RNA (Reverse Transcription),
9. Polymerase Chain Reaction from cDNA (Reverse Transcription-PCR)
10. Agarose gel electrophoresis of PCR product
11. Total protein extraction from human cancer cell lines
12. Protein estimation
13. SDS-PAGE electrophoresis of extracted proteins
14. Coomassie blue staining of protein gel
15. MTT Assay for cell proliferation/cytotoxicity
Only 12 seats per batch so please register early to confirm your participation. We will update on the website when seats are full in a batch. Each participant will do all techniques real hands-on.
Registration Fees (food & accommodation is not included):
For Students: Rs. 11000/- (student id copy must be emailed)
For Faculty/Doctors/Scientists/Postdocs: Rs. 15000/-
Registration fee is non-refundable. If seats are not available then it will be refunded.
Methods of Registration:
Please download and fill the Cell Culture Workshop Registration Form and email (scan or photo) it to email@example.com along with the proof of payment by any of the following payment methods:
NEFT Transfer (or SWIFT Transfer for International candidates):
You can pay directly in our bank account by NEFT (Indian) or SWIFT (International) transfer. For bank account details please click on the following link: Bank Account Details
Payment by Google Pay:
You can pay to Mobile No. 9545089202 or Google Pay UPI Id: sheomohansingh-1@okhdfcbank
Payment by Paytm or Paytm UPI:
You can pay to Paytm No. 9545089202 or Paytm UPI Id: 9545089202@paytm
Payment by PhonePe App:
You can pay to UPI ID sheomohan1@ybl by using PhonePe App on your mobile phone.
WORKSHOP CERTIFICATE will be issued to all participants on last day.
Bachelors, Masters and PhD students, medical students, doctors, lecturers, scientists, industry members in the field of Biological Sciences, Biotechnology, Bioinformatics, Life Sciences, Medical Sciences, Pharmaceutical Sciences, Chemical Sciences and related subject areas who are interested in learning hands-on laboratory techniques.
Accommodation should be booked in nearby PG hostels/guesthouses or hotels directly by the candidate. For more information and any help in finding accommodation please email to firstname.lastname@example.org.
For enquiries please email or call:
Tel No: +91-7798490002