1-Week Hands-on Workshop on Human/Cancer Cell Culture Techniques & MTT Assay, Pune, India
Venue: ICSCCB, R.H. 2, Ujwal Regalia, Near Prabhavee Tech Park, Baner Road, Pune – 411045, India
This is 1-Week hands-on workshop on the following techniques using human embryonic kidney cell line and human blood cancer cell lines:
- Animal cell culture techniques, passaging and maintenance of cell lines
- Media preparation
- Cryopreservation (freezing and thawing of cell lines)
- Genomic DNA isolation from cancer cell lines,
- DNA estimation
- DNA agarose gel electrophoresis
- Total RNA extraction
- cDNA synthesis from RNA (Reverse Transcription),
- Polymerase Chain Reaction from cDNA (Reverse Transcription-PCR)
- Agarose gel electrophoresis of PCR product
- Total protein extraction
- Protein estimation
- SDS-PAGE electrophoresis of extracted proteins
- Coomassie staining of protein gel
- MTT Assay for cell proliferation/cytotoxicity
This is a basic workshop intended for beginners to teach and train them in animal cell culture and molecular techniques using the human/cancer cell lines for various experiments. It will be supported by some lectures on basic cell culture techniques, media preparation, gene/protein expression & interaction networks in hematopoietic stem cells (HSCs) and cancer cells.
Prof. Dr. Sheo Mohan Singh
MSc(UK), PhD(Germany), PDF(USA,UK)
Director, ICSCCB, Pune, India
Only 10 seats per batch so please register early to confirm your participation. We will update on the website when seats are full in a batch. Each participant will do all techniques real hands-on.
Registration Fees (food & accommodation is not included):
For students studying in India: Rs. 11000/- (US$ 200 for International candidates).
For working/faculty/scientists/industry in India: Rs. 16000/- (US$ 300 for International Candidates)
Note: You must email your scanned student ID card to register under students category.
Registration fee is non-refundable. However, if seats are not available then it will be refunded or next available workshop date can be given.
Other Methods of Registration:
Please download and fill the Cell Culture Workshop Registration Form and email (scan or photo) it along with the proof of payment by any of the following payment methods:
NEFT Transfer (or SWIFT Transfer for International candidates):
You can pay directly in our bank account by NEFT (Indian) or SWIFT (International) transfer. For bank account details please click on the following link: Bank Account Details
Payment by Google Pay:
You can pay to Mobile No. 9545089202 or Google Pay UPI Id: sheomohansingh@okhdfcbank
Payment by Paytm or Paytm UPI:
You can pay to Paytm No. 9545089202 or Paytm UPI Id: 9545089202@paytm
Payment by BHIM App:
You can pay to UPI ID 9545089202@upi by using BHIM App on your mobile phone.
Payment by PhonePe App:
You can pay to UPI ID 9545089202@ybl by using PhonePe App on your mobile phone.
WORKSHOP CERTIFICATE will be issued to all participants on last day.
11/12th, UG/PG/PhD students, faculty, scientists as well as people working in industry in the field of Biotechnology, Bioinformatics, Life Sciences, Medical Sciences, Pharmaceutical Sciences, Chemical Sciences and related subject areas.
Accommodation should be booked in nearby PG hostels/guesthouses or hotels directly. Please click the following link to download accommodation information. For more information and any help in finding accommodation please contact on +91-7798490002 or email to firstname.lastname@example.org.
For enquiries please email or call:
Tel No: +91-7798490002