Summer Training

Biotech and Animal Cell Culture Summer/Short Training
(Rolling Advert, can be started on mutually agreed dates anytime of the year)
(1 Month, Full-time, Regular Program)

Number of seats:  8

Next batch starting on:  18 June 2018  and 25 June 2018

Venue: ICSCCB, R.H. 2, Ujwal Regalia, Near Prabhavee Tech Park Building, Baner Road, Pune – 411045, India

Details of techniques to be learnt during the program:
Molecular Biology and Cell Culture Lab:
Restriction digestion
DNA gel electrophoresis
DNA gel extraction
Preparation of competent cells
Transformation and plating on LBA plates
Plasmid DNA isolation
Genomic DNA isolation
Polymerase Chain Reaction (PCR)
Animal Cell Culture and maintenance of human cancer cell lines
Freezing & thawing of human cancer cell lines
RNA extraction from human cancer cell lines
cDNA synthesis
Reverse Transcription PCR (RT-PCR)
Induction of proliferation and differentiation in human cancer cell lines
Protein extraction from human cancer cell lines
SDS-PAGE and coomassie blue staining
MTT Assay for proliferation/cytotoxicity
Cytospin slide preparation and staining of hematopoietic cells for visualizing differentiation of cells

Bioinformatics Lab:
DNA sequence analysis
Protein sequence analysis
Protein structure visualization
Bioinformatics databases
PCR primer designing
Gene cloning
Promoter analysis
Genomics Data Visualization: browsing and comparing human and mouse genomic data with the help of NCBI Genome viewer and UCSC Genome browser

Prof. Dr. Sheo Mohan Singh
MSc(UK), PhD(Germany), PDF(USA,UK)
Director, ICSCCB, Pune, India

Only 8 seats are available so please register early to confirm your participation. We will update on the website if the batch is full. Each participant will do all techniques real hands-on.

Registration Fees (food & accommodation is not included):

For 1 month:  Rs. 18,000/-  (Seats available, Click here for online payment)
For 2 months: Rs. 25,000/-  (Seats available, Click here for online payment)

Registration fee is non-refundable. However, if seats are full then it will be refunded or next available workshop date can be given.

Payment by BHIM App:
You can pay to UPI ID 9545089202@upi by using BHIM App on your mobile phone. Click here for more info on BHIM App. It’s free.

Payment by PhonePe App:
You can pay to UPI ID 9545089202@ybl by using PhonePe App on your mobile phone. Click here for more info on PhonePe App. It’s free.

CERTIFICATE will be issued to all participants on last day.

UG/PG students in the field of Biotechnology, Bioinformatics, Life Sciences, Medical Sciences, Pharmaceutical Sciences, Chemical Sciences and related subject areas.

Offline Registration:
If you want to pay by cash or NEFT then please download and fill the Application Form and send it along with proof of payment by post or send scanned copy by email to

Accommodation Info:
Accommodation should be booked in nearby PG hostels/guesthouses and hotels directly. Please click the following link to download accommodation information. If you need help then please call on +91-7798490002 or email to

For enquiries please email:
Tel No: +91-7798490002