Summer Training

Biotech and Animal Cell Culture Summer Training (Rolling Advert, can be started on mutually agreed dates anytime)
(Two Months, Full-time, Regular Program)
Number of seats:  8

Venue: ICSCCB, R.H. 2, Ujwal Regalia, Near Prabhavee Tech Park Building, Baner Road, Pune – 411045, India

Details of techniques to be learnt during the program:
Molecular Biology and Cell Culture Lab:
Restriction digestion
DNA gel electrophoresis
DNA gel extraction
Preparation of competent cells
Transformation and plating on LBA plates
Plasmid DNA isolation
Genomic DNA isolation
Polymerase Chain Reaction (PCR)
Animal Cell Culture and maintenance of human cancer cell lines
Freezing & thawing of human cancer cell lines
RNA extraction from human cancer cell lines
cDNA synthesis
Reverse Transcription PCR (RT-PCR)
Induction of proliferation and differentiation in human cancer cell lines
Protein extraction from human cancer cell lines
SDS-PAGE and coomassie blue staining
MTT Assay for proliferation/cytotoxicity
Cytospin slide preparation and staining of hematopoietic cells for visualizing differentiation of cells

Bioinformatics Lab:
DNA sequence analysis
Protein sequence analysis
Protein structure visualization
Bioinformatics databases
PCR primer designing
Gene cloning
Promoter analysis
Genomics Data Visualization: browsing and comparing human and mouse genomic data with the help of NCBI Genome viewer and UCSC Genome browser

Prof. Dr. Sheo Mohan Singh
MSc(UK), PhD(Germany), PDF(USA,UK)
Director, ICSCCB, Pune, India

Only 8 seats are available so please register early to confirm your participation. We will update on the website if the batch is full. Each participant will do all techniques real hands-on.

Registration Fees (food & accommodation is not included):

Rs. 25000/-  (for persons residing in India)
US$ 500/-  (for NRIs/International candidates residing outside of India).

Registration fee is non-refundable. However, if seats are not available then it will be refunded or next available workshop date can be given if candidate wants.

CERTIFICATE will be issued to all participants on last day.

UG/PG students in the field of Biotechnology, Bioinformatics, Life Sciences, Medical Sciences, Pharmaceutical Sciences, Chemical Sciences and related subject areas.

Payment for Registration:
Please pay online by clicking this link Online Payment or download the Application Form and send it along with a Demand Draft in favor of ‘Agamya Biotech Private Limited’ payable at ‘Pune’  to the address given on the form.

Payment by Cash Deposit or Online NEFT Transfer:
You can also pay by direct deposit of Cash/Cheque in your city in any branch of Oriental Bank of Commerce or transfer online by net-banking/NEFT from any bank. Bank account details are available here:  Cash Deposit/Bank Transfer details. Please email the Application Form along with payment receipt/ref number to confirm your registration.

Accommodation Info:
Accommodation should be booked in nearby hotels/PG guesthouses directly. Please click the link Accommodation Information to download the details. If you need help then please call Mr. Lakshmikanth Reddy at ICSCCB on +91-7798490002 or email to

For enquiries please email:
Tel No: +91-7798490002