Summer Training

Biotech, Animal Cell Culture and Cancer Biology Summer Training, at the ICSCCB, New Delhi.
(1 Month, Full-time, Regular Program)

You can check lab photos on our posts at: and

Prof. Dr. Sheo Mohan Singh
MSc(UK), PhD(Germany), PDF(USA,UK,Germany)
Director, ICSCCB, New Delhi, India

Number of seats:  12 (Register early as seats get filled before the summer because of high quality and individual training)

Summer Training dates for 2024 (Timings 10:00am-6:00pm):
01 July – 30 July 2024    (1 Seat Available)
15 July – 14 Aug 2024    (4 Seats Available)
01 Aug – 30 Aug 2024    (Seats Available)

Venue: ICSCCB, First Floor, Khasra 132, Plot 4, IGNOU Main Road, Near Saint Robin Public School, Neb Sarai, Saket, New Delhi – 110068, India (Nearest Metro Station is Saket on the Yellow Line)

Details of techniques (done hands-on by each candidate) to be learnt during the program:

Molecular Biology and Cell Culture Lab:
1. MTT Assay for cell proliferation/cytotoxicity
2. Cell culture techniques, passaging and maintenance of human cancer cell line
3. Media preparation and counting of cells
4. Cryopreservation: Freezing & thawing of human cancer cell line
5. Genomic DNA isolation from cancer cell line,
6. Genomic DNA estimation
7. Genomic DNA agarose gel electrophoresis
8. Total RNA extraction from human cancer cell line
9. cDNA synthesis from RNA (Reverse Transcription),
10. Polymerase Chain Reaction from cDNA (Reverse Transcription-PCR)
11. Agarose gel electrophoresis of PCR product
12. Total protein extraction from human cancer cell line
13. Protein estimation
14. SDS-PAGE electrophoresis of extracted proteins
15. Coomassie blue staining of SDS-PAGE gel
16. Induction of proliferation and differentiation in human cancer cell line
17. Cytospin slide preparation of hematopoietic cells
18. May-Gruenwald Giemsa Staining of cytospin slides of hematopoietic cells and visualizing morphology of differentiating cells
19. Plasmid DNA isolation
20. Plasmid DNA gel electrophoresis
21. Plasmid DNA gel extraction
22. Restriction digestion
23. Ligation
24. Agarose gel electrophoresis of restriction digested and ligated DNA
25. Preparation of competent cells
26. Transformation and plating on LBA plates

Bioinformatics tools for the above techniques:
27. DNA databases
28. Sequence Analysis: Pairwise sequence alignment, Multiple sequence alignment, BLAST tools
29. Designing of PCR primers,
30. Restriction analysis, restriction maps, plasmid maps,
31. Searching for homologous and paralogous sequences,
32. Promoter analysis, Transcription factor databases,
33. Whole genome data visualisation: browsing genomic data with the help of NCBI Genome viewer or UCSC Genome browser.

This program is designed for beginners of all ages who wants to become confidant in lab techniques. This is a practical hands-on program to train the candidates in animal cell culture, molecular biology techniques and MTT assay using the human embryonic and cancer cell lines for various experiments. It is supported by lectures on cell culture techniques, molecular biology assays, MTT assay, gene/protein expression/interaction networks in hematopoietic stem cells (HSCs) and cancer cells.

Only 12 seats are available so please register early to confirm your participation. We will update on the website if a batch is full. Each participant will do all techniques real hands-on.

Registration Fees (food & accommodation is not included):   
Rs. 21000/- + Rs. 3600 GST (Total Rs. 24600). Same fees for International candidates.

Registration fee is non-refundable (because of limited seats to maintain the high quality of training). If we cannot provide the seat then the fees will be refunded. One time date change will be allowed under special circumstances.

Methods of Registration:
After depositing the fees by payment methods below, please fill the following online application form: and upload the payment proof/screen shot in the form. If there is any problem in filling the form, contact us by email:

Bank Transfer: You can pay to following bank account:
Account name: International Centre for Stem Cells CAB
Bank name: HDFC Bank, Aundh Branch
IFSC Code:HDFC0000052
MMID: 9240137
Account type: Current Account
Account number: 50200032963132

Payment by any UPI method e.g. Google Pay, PhonePe, Paytm etc:
You can make payment to the following ICSCCB UPI Id: 9545089202@upi

CERTIFICATE will be issued to all participants on last day.

Current or past Students of UG, PG and PhD programs, medical students, doctors, lecturers, scientists, industry members in the field of Biological Sciences, Biotechnology, Bioinformatics, Life Sciences, Medical Sciences, Pharmaceutical Sciences, Chemical Sciences and related subject areas who are interested in learning hands-on laboratory techniques.

Accommodation Info:
Accommodation should be booked in nearby PG hostels/guesthouses and hotels directly by the candidate. If you need help then please email to

For enquiries please email or call:
Dr. Sheo Mohan Singh
Tel No: +91-7798490002