Dissertation Projects

Dissertation Projects
(4 – 6 Months, Full-time, Regular Program)

Venue: ICSCCB, Kh. No. 132, Plot 4, IGNOU Main Road, opposite Hero Motors Service Centre, Neb Sarai, New Delhi – 110068, India (Closest Metro Station Saket on Yellow Line)

You can check lab photos on our posts at: https://www.linkedin.com/company/icsccb and https://www.linkedin.com/in/dr-sheo-mohan-singh

Number of seats:    8

Next Batch starting:    Mid-January and Mid-July every year (Rolling applications accepted)

Broad Topics:
Proliferation and differentiation of myeloid cells and Regulation of myeloid transcription factors and other proteins in hematopoiesis and their dysregulation in leukemic cancers.

Details of techniques (hands-on by each candidate) to be learnt during the program:

Molecular Biology and Cell Culture Lab:
1. Cell culture techniques, passaging and maintenance of human cancer cell lines
2. Media preparation and cell counting
3. Cryopreservation: Freezing & thawing of human cancer cell lines
4. Genomic DNA isolation from cancer cell lines,
5. Genomic DNA estimation
6. Genomic DNA agarose gel electrophoresis
7. Total RNA extraction from human cancer cell lines
8. cDNA synthesis from RNA (Reverse Transcription)
9. Standard Polymerase Chain Reaction from cDNA (Reverse Transcription-PCR)
10. Agarose gel electrophoresis of PCR product
11. Induction of proliferation and differentiation in human cancer cell lines
12. Total protein extraction from human cancer cell lines
13. SDS-PAGE electrophoresis of extracted proteins
14. Coomassie blue staining of SDS-PAGE gel
15. MTT Assay for proliferation/cytotoxicity
16. Cytospin slide preparation of hematopoietic cells
17. May-Gruenwald Giemsa Staining of cytospin slides of hematopoietic cells and visualizing morphology of differentiating cells
18. Plasmid DNA isolation
19. Plasmid DNA gel electrophoresis
20. Plasmid DNA gel extraction
21. Restriction digestion
22. Ligation
23. Agarose gel electrophoresis of restriction digested and ligated DNA
24. Preparation of competent cells
25. Transformation and plating on LBA plates
26. Standard Polymerase Chain Reaction (PCR)
27. Agarose gel electrophoresis of PCR product

Bioinformatics Part:
28. DNA sequence analysis: Pairwise sequence alignment, Multiple sequence alignment, BLAST tools
29. Protein sequence analysis
30. Protein structure visualization
31. Bioinformatics databases
32. PCR primer designing
33. Promoter analysis
34. Whole genome data visualisation: browsing data with the help of NCBI Genome viewer or UCSC Genome browser.

Current students in their final semester for dissertation project (Bachelors/Masters) or PhD students who want to do a part of their dissertation project at ICSCCB.

Special Features:

  1. High emphasis on practical training during the program.
  2. All students would participate and then help in conducting the Biotech and Cell Culture workshops organized by ICSCCB.
  3. All students would participate and help in organizing the International Conference on Stem Cells and Cancer (ICSCC) (http://www.icscc.in/).
  4. All students would participate and help in organizing the International Conference on Biotechnology and Bioinformatics (ICBB) (http://www.icbb.in/).
  5. We will arrange a special exclusive interaction between our students and the key invited speakers at the above conferences.

Program starts in:     Mid-January and Mid-July every year. Please mention your preferred date of starting in the application. Applications are accepted the whole year round.

Laboratory Fees (Non-refundable):
Rs. 60,000/-
(Accommodation charges are separate and must be arranged by candidates.)

Application procedure:
Please download and fill the Application Form and send it along with copies of your degree certificates/mark sheets and CV by post or scanned copy by email to icsccb2012@gmail.com.

You will be informed of the admission decision once we receive complete application along with copies of degree certificates/mark sheets and CV.

Payment of program fees:
After receiving admission offer letter, you should pay the  entire program fees by direct deposit of cash/cheque in your city or by any of the following payment methods:

Bank Transfer: You can pay to following bank account:
Account name: International Centre for Stem Cells CAB
Bank name: HDFC Bank, Aundh Branch
IFSC Code:HDFC0000052
MMID: 9240137
Account type: Current Account
Account number: 50200032963132

Payment by any UPI method e.g. Google Pay, PhonePe, Paytm etc:
You can make payment to the following ICSCCB UPI Id: 9545089202@upi





Accommodation Info:
Accommodation should be booked in nearby PG hostels/guesthouses or hotels directly by the candidate. For more information and any help in finding accommodation please contact on +91-7798490002 or email to icsccb2012@gmail.com.

For any queries please email to: icsccb2012@gmail.com